Sialic Acid (n-acetylneuraminic Acid) in Human Serum.

نویسندگان

  • J A CABEZAS
  • J V PORTO
چکیده

A simple spectrophotometric method is described for the sialic acids which utilizes the reaction with resorcinol and extraction with butyl acetate-n-butanol as a solvent. Normal values for adults, utilizing N-acetylneuraminic acid as a standard, were found to be 60 ± 10.4 mg./100 ml. Values for newborn babies averaged 40.4 ± 5.7. Similar values were found for specimens from 2-month-old infants to 27-year-old adults. Higher values were noted in the case of rickets, severe burns, rheumatic disease, and tuberculosis.

برای دانلود متن کامل این مقاله و بیش از 32 میلیون مقاله دیگر ابتدا ثبت نام کنید

ثبت نام

اگر عضو سایت هستید لطفا وارد حساب کاربری خود شوید

منابع مشابه

Evidence for a sialic acid salvaging pathway in lepidopteran insect cells.

We previously described a transgenic insect cell line, Sfbeta4GalT/ST6, that expresses mammalian beta-1,4-galactosyltransferase and alpha2,6-sialyltransferase genes and produces glycoproteins with terminally sialylated N-glycans. The ability of these cells to produce sialylated N-glycans was surprising because insect cells contain only small amounts of sialic acid and no detectable CMP-sialic a...

متن کامل

A transgenic insect cell line engineered to produce CMP-sialic acid and sialylated glycoproteins.

We have previously engineered transgenic insect cell lines to express mammalian glycosyltransferases and showed that these cells can sialylate N-glycoproteins, despite the fact that they have little intracellular sialic acid and no detectable CMP-sialic acid. In the accompanying study, we presented evidence that these cell lines can salvage sialic acids for de novo glycoprotein sialylation from...

متن کامل

Human-Specifi c Changes in Sialic Acid Biology

CMAH CMPN -acetylneuraminic acid hydroxylase CMP-Kdn Cytidine monophospho-2-keto-3 deoxynonulosonic acid CMP-Neu5Ac Cytidine monophosphoN -acetylneuraminic acid CMP-Neu5Gc Cytidine monophosphoN -glycolylneuraminic acid Gal Galactose GlcNAc N -Acetylglucosamine ITIM Immunoreceptor tyrosine-based inhibitory motif Kdn 2-Keto-3 deoxynonulosonic acid Neu5Ac N -Acetylneuraminic acid Neu5Gc N -Glycoly...

متن کامل

Study of Adenosine Deaminase and Serum Protein Bound Sialic Acid Levels in Alcoholic Liver Disease

Alcohol liver disease Adenosine deaminase Protein bound sialic acid Inflammation

متن کامل

Evaluation of Serum Sialic Acid Levels in Head and Neck Malignancies

Head and neck cancers (HNC) have emerged as a leading cause of cancer related mortality and morbidity worldwide. Especially in developing countries, due to a lack of awareness and healthcare facilities, most HNC cases are diagnosed at advanced stages. Currently, there are no reliable non-invasive diagnostic modalities to detect HNC and follow up treated cases. Serum sialic acid is a promising c...

متن کامل

ذخیره در منابع من


  با ذخیره ی این منبع در منابع من، دسترسی به آن را برای استفاده های بعدی آسان تر کنید

برای دانلود متن کامل این مقاله و بیش از 32 میلیون مقاله دیگر ابتدا ثبت نام کنید

ثبت نام

اگر عضو سایت هستید لطفا وارد حساب کاربری خود شوید

عنوان ژورنال:
  • Clinical chemistry

دوره 10  شماره 

صفحات  -

تاریخ انتشار 1964